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Tancet notes: Chemistry : Chromatography

By : Engineer
On : 00:32
In :

Chromatography may be defined as, “an analytical technique for identification and separation of various components of a mixture on the basis of difference in their affinity for stationary and mobile phases”.

 The mixture is applied to either a solid or liquid phase called stationary phase
 Liquid or gas phase is allowed to move slowly over the stationary phase , called mobile phase.
Types of chromatography:

  Chromatographic process may be classified as 

1.    Adsorption chromatography
2.    Partition chromatography.
3.    Ion- exchange chromatography.


               Large volume of samples can be separated.

             Separation of a mixture of similar molecules is possible only in adsorption in adsorption chromatography.


Purification of substances from their contaminants

Identification of products


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